... of these stages were identified. "Taxonomy to Function" associations were identified for all the major taxa found in the SWD fluids. WGS was performed on positive Sulfate Reducing Bacteria (SRB) and Acid Producing Bacteria (APB) media bottles inoculated in the field for a Flowback sample. Four of the six major...

... Abstract Hydraulic fracturing presents an ideal breeding ground for microbial proliferation due to the use of large volumes of nutrient-rich, water-based process fluids. Bacteria and/or archaea, when left uncontrolled topside or in the reservoir, can produce hydrogen sulfide, causing biogenic...

... Abstract New biochemically-derived products for the removal of paraffin wax from oil wells do not require additional capex nor heat and do not utilize bacteria. They contain inactivated microbial cells, biosurfactants and biosolvents, and other components harvested as microbial byproducts...

... Abstract Microbial-induced problems in oil and gas incur high costs and cause severe environmental and safety concerns. Most of these problems are directly caused by surface-adhered bacteria colonies known as biofilms. Distinct populations of bacteria within a biofilm can symbiotically alter...

... not create halogenated organics and does not introduce oxygen into the system. Performic acid provides unique advantages for use in many energy related applications, particularly in water treatment. Treatment using performic acid, at 0.5ppm, provides a microbial reduction equivalent to 3 logs of bacteria/mL...

... society of petroleum engineers monitoring microbial control concentration biocide regime microbial microorganism bacteria Upstream Oil & Gas regime active ingredient adenosine triphosphate biocide evaluation Introduction Biocides are commonly used in the oil and gas industry...

..., cationic biocides rapidly associated with shale preferentially over water, significantly reducing the availability of these compounds in the aqueous phase. Accordingly, their efficacy against planktonic bacteria substantially diminished. Across the range of shale types (surface area and reservoir source...

... constituent formation damage framboid mineral time series shale plate shale oil oil shale Eagle Ford shale specimen shale mass pyrite hydraulic fracturing concentration experiment Transport Mechanism bacteria electrode Introduction Our journey of shale has continued over several...

... sewage! Organic carbon is a potential energy source for bacteria, and, as such, is a macro-indicator of water quality. The presence of TOC in the form of Assimilable Organic Carbon (AOC) is the primary food source of bacteria. Oilfield bacteria can be detrimental to the quality and performance...

... Corrosion riser corrosion enhanced recovery well integrity major component composition injection system bacteria materials and corrosion Upstream Oil & Gas classification water injection system amorphous deposit hydrocarbon mixed deposit schmoo substance corrosion inhibitor magnetite...

... sequences (unidentifiable at the genus level because no similar DNA sequences are present in the global database) ranged from 1.8% to 25.8%. Of the bacteria that could be identified, a high percentage of halophilic (salt loving) bacteria, and of SRB were observed in all samples. These results demonstrate...

... measures aimed at killing bacteria downhole may be appropriate for these shale oil but not for these shale gas environments. hydraulic fracturing oil field shale gas concentration souring monitor souring Sulfide salinity determination SRB Upstream Oil & Gas source water corrosion...

... control of microbial contamination in these applications is required for the sustained quality of the production fluids and structural assets. The microbial groups of concern are sulfate reducing bacteria (SRB), acid-producing bacteria (APB), and facultative anaerobes. In this study, representative...

... Abstract Nitrate can control biogenic souring by lowering sulfate reducing bacteria (SRB) metabolic activity and shifting the microbial community such that nitrate-reducing bacteria (NRB) out-compete SRB for nutrients. Nitrate was applied in a laboratory study using a 20-cc packed bed upflow...

... Abstract A reservoir souring forecasting model was presented previously. This model utilized a generic algorithm to determine, via history match, the extent of H 2 S biogeneration by sulfate-reducing bacteria (SRB) within the waterflooded reservoir such that the production to the surface could...

... and THPS-based products were tested against planktonic halophilic sulfate reducing bacteria (SRB's) using high throughput testing. No differentiation between products was observed under these conditions. More sophisticated test methods that evaluate kill of sessile bacteria again, showed little...

... of this biocide would not persist beyond a few days. Industry standard techniques (NACE Std. TMO194-94) for quantifying bacteria were applied to water samples taken during the job and over several weeks of production. The biocide was also evaluated for compatibility with common fracturing additives and for its...

... Abstract Gas production from subsurface shales requires fracture technologies in which fracturing fluid, consisting of guar gum-suspended sand, is forced into the fractures to "prop" them open. The guar gum is easily degraded by bacteria both downhole and at the surface, compromising water...

... and fatty acids in the formation water in the presence of sulfate-reducing bacteria (SRB). There are proposed mechanisms that describe the effect of nitrate injection on souring remediation. Depending on the circumstances, more than one mechanism may occur at the same time. These mechanisms include...

... of petroleum engineers biocide bacteria microbial control program microbial count operation reduction reservoir conditions glutaraldehyde molecular microbiology Introduction Biocides are routinely used as chemical additives for hydraulic fracturing operations in order to prevent biogenic H2S...