As the oil and gas industry has been investigating the challenges in identifying reservoir compartmentalization and interpreting the negative influences it generates, diverse disciplines within the oil and gas industry have presented beneficial approaches to inspect and reduce the challenges found while exploring and appraising oil fields. despite the various pieces of literature that have been published in this field stating that reservoir compartments are either structurally or stratigraphically in origin depending on the depositional facies or fault geometry. This study presents, geochemical data from twenty oil samples which are used to investigate horizontal and vertical compartmentalization of the Jawdah Field, which is located in the south of Oman. The data were intended to identify the causes of the segmentation using fluid properties. The results from each oil sample are integrated with carbon isotopes, whole-oil gas chromatography, saturates, aromatics, resins and asphaltenes (SARA) analysis and biomarkers in order to detect different variations in fluid composition that may be lead to better understanding of reservoir compartmentalization. Based on these four analyses, the research findings indicate that Jawdah oils are derived from a single source rock (Huqf oils). The identification of the Huqf source rock was based on the similarities between pristane and phytane peaks from the whole-oil gas chromatography data and the carbon isotopes for the Jawdah oils and from the Grantham (1988) paper. However, the API gravities in Jawdah field varied ranging from light to moderately heavy oil (20-33 API), indicating a possibility of other geological factors affected the alteration of the crude oil. Therefore, these oils were further characterized by interpreting the level of maturation and biodegradation ratios using biomarker signatures. This combination of methods has provided a more advanced method of investigating and interpreting compartmentalized reservoirs and it will lead to enhancing recovery. It was found that the Gharif and Al Khlata reservoir units are not in communication, potentially impacting well design, and field development planning.