Most laboratory work in MEOR, both in screening and in development, is performed under in situ temperatures and in brines similar to those of the target reservoir. However, the effect of in situ pore pressures in such work is normally ignored. Much of our understanding of the effects of hydrostatic pressure on microorganisms comes from studies of microbial communities at the ocean floor. Since nearly 90% of the ocean floor has pressures ranging from 10 MPa to over 100 MPa, there is a paucity of data concerning effects on microorganisms at the lower pressures found in many oil reservoirs.

In our studies of the indigenous cells from injection brine at the North Burbank Unit, we have found that pressures ranging from 0.1 MPa to 8.8 MPa can have a significant effect on pH changes, substrate utilization, end product formation and permeability reduction. In general, hydrogen ion concentrations and carbohydrate utilization rates increased with increasing pressures. Methanol was found to be a significant end-product in the presence of hydrostatic pressure but found to be absent under atmospheric conditions. The fact that some of the observed effects could be demonstrated by simply reducing the gas headspace suggests that gas solubility could be an important factor. Gas formation in a core was estimated to account for as much as 50% of the permeability decrease observed in cores run under atmospheric pressure. Therefore the changes observed are of such magnitude as to alter the MEOR process in the reservoir from that indicated by laboratory studies not done under pressure.

Our results strongly suggest that all MEOR studies pertaining to a known reservoir should be evaluated under the in situ conditions of the reservoir, including pressure.

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