In addition to geological and petrophysical data acquisition during the exploration stage, in-situ fluid analysis provides a wealth of information for the appraisal of new discoveries. A recently introduced wireline sampling tool incorporating a downhole fluid analyzer is capable of analyzing fluid composition in real time at downhole conditions, and of measuring the fluorescence spectra of crude oils. These new measurements provide valuable information necessary for the identification and validation of reservoir structures that define the distribution of fluids in the accumulation. The relevance of high quality fluid data in the early stages of the producing life of the reservoir is widely recognized. We present field results of the application of the new sampling tool in an exploration well, where a composition gradient was detected along a 30m liquid hydrocarbon column grading from a 45API crude on the top to a 33API crude on the bottom. During the sampling of the gas cap, retrograde dew formation was detected identifying the fluid and identifying valid sampling conditions. This new information was used to modify the sampling program. Fluid composition analysis relies on optical absorption methods and is currently capable of providing the mass fraction of three hydrocarbon molecular groups: C1, C2-5 and C6+, and CO2. We perform fluorescence spectroscopy by measuring light emission in the green and red ranges of the spectrum after excitation with blue light. Fluorescence in this range is related to the concentration of polycyclic aromatic hydrocarbons (PAH’s) in the crude oil. Using the pump-out module to segregate different fluid phase enhances phase detection with fluorescence.