A series of fundamental laboratory tests was performed to develop a novel conceptual grout, that improves the sealability of soil and rock using microorganisms, hereafter denoted as biogrout. Biogrout, which includes calcium ions and glucose, is a material to produce the precipitation of calcium carbonate (CaCO3), which plugs voids of rock and soil and, moreover, cracks of rock. In our previous studies, precipitation tests on CaCO3), were carried out at 25°C. This paper especially focuses on evaluating the influence of the temperature on the precipitation of CaCO3), to consider applications of biogrout in soils of various areas with varying climates. In laboratory tests, biogrout was added to soils from vari-ous areas in Japan under wide-ranging temperatures (5 to 35°C), and the results showed that the tem-perature highly affected the precipitation of CaCO3), because of the temperature dependence of microbial metabolism.

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